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Vitamin D supplementation in health and latent tuberculosis promotes regulatory T cell expression
PDPI Jatim, 02 Mar 2018 14:01:35

Gemma Hawthorne1, Zhang JinJin1, David Thickett1 and Alice Turner1
1Clincal and Experimental Medicine, University of Birmingham, Birmingham, United Kingdom

 

Background: Vitamin D acts as an immune modulator. Its effect in mycobacterial infection may be considered in future therapeutic approaches. Cytotoxic T lymphocyte antigen (CTLA4) is involved in T cell tolerance and its expression rises with T cell receptor activation.

Methods: CTLA-4 / FoxP3 expression and the effect of vitamin D supplementation in health and latent TB was measured using flow cytometry. Cells were stimulated with (Staphylococcal Enterotoxin B) SEB or Purified Protein Derivative (PPD) and the effect of either 1,25(OH)2D3 or 25(OH)D3 on the above parameters was recorded.


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Results:
There is no significant difference in the frequency of T reg markers /T reg numbers ex vivo in health or latent TB (p>0.05). Vitamin D increases T regulatory cell frequency and there is a statistically significant rise in the CTLA4 MFI in SEB stimulated cells with vitamin D supplementation (p=0.001). The response in latent TB is blunted, with significantly different values between health and disease (p<0.05).

Conclusion: Vitamin D enhances the expression of both T reg markers and CTLA4 immune regulatory receptor in both health and latent TB and is suggestive of the immune regulatory role of vitamin D